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Lonza cord blood CD34 HLA cells (2C-101H) contain 1 million hematopoietic stem and progenitor cells and are ideal for studies relating to transplantation and stem cells They are also widely used in humanized mouse model development Cord blood derived CD34 HLA cells (2C-101H) is available with HLA information enabling prescreening and selection of lots most appropriate for your study providing cost and time savings HLA information eliminates the need to use cells for obtaining HLA genotype increasing the number of cells that can be used within your experiment CD34 HLA cells are ethically sourced and are isolated using Institutional Review Board (IRB) or other regulatory authority-approved consent forms and protocols Large inventory supports stable supply from a single vendor Guarantee Lonza guarantees the performance of Cells only if appropriate Lonza recommended Media and Reagents are used exclusively and the recommended storage and use protocols are followed
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Mobilized peripheral blood CD34 hematopoietic stem and progenitor cells are positively selected using a immunomagnetic CD34 microbead labeling system Cells are formulated in Lonza Fresh Media and shipped in a cryovial Cell purity is determined using a FACS assay Count and viability is determined using AO/PI Cells are collected from healthy donors following IRB protocols
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CD34+ Stem/Progenitor cells are positively selected from Regimen F mobilized peripheral blood leukapheresis using immunomagnetic CD34+ microbeads. CD34+ cells are shipped in a 2mL cryovial. Purity is determined prior to cryopreservation and is ≥ 90%. Viability is ≥ 80% post-thaw. 1 Million Cells per vialAllCells is the industry leader in supplying mobilized Leukopaks with the shortest lead time on the market. We offer three different mobilization regimens: G-CSF (Neupogen)- plerixafor (Mozobil)- and a combination of the two drugs.
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Mobilized peripheral blood CD34 hematopoietic stem and progenitor cells are positively selected using a immunomagnetic CD34 microbead labeling system Cells are cryopreserved in Lonza Cryo Media in a cryovial Cell purity is determined post thaw using a FACS assay Count and viability is determined using hemacytometer (trypan blue method) Cells are collected from healthy donors following IRB protocols
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Cord blood from a two or more donors is used to positively select CD34+ cells using an immunomagnetic labeling system. Stem/Progenitor cells have the potential to self-renew and differentiate into all the mature blood cell lineages. The cells are cryopreserved with CS10 in a cryovial. Stem cell purity is determined post thaw using a FACS assay. Cell count and viability are determined using AO/PI. Cells are collected from healthy donors following IRB protocols. Umbilical Cord Blood is a rich source of primitive CD34+ hematopoietic stem and progenitor cells reported to be more naive with a higher proliferative- engraftment and multipotent potential than those isolated from adult bone marrow. Because of these attributes- cord blood can be a great tool for applications where bypassing HLA mismatch is required. AllCells offers cryopreserved cord blood CD34+ from both single cord or multiple/mixed cord donor samples.
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Normal Human Dermal Fibroblasts-Neonatal, Xeno-Free, when grown in Lifeline's FibroLife Xeno-Free Complete Medium, provide an ideal xeno-free culture system to establish xeno-free human feeder layers for human embryonic stem cell cultures.
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